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Effects on dopamine neuronal survival and function were assessed by anti-TH immunostaining and negative geotaxis assays.

The UAS-Gal4 system was employed for cell-type specific expression of the various transgenes. In this study, we examined how PINK1/parkin mediated regulation of the pathogenic substrate PARIS impacts dopaminergic mitochondrial network homeostasis and neuronal survival in Drosophila. Mitochondrial functions of PINK1 and parkin are also tied to their proteasomal regulation of specific substrates. Although PINK1 and parkin are known to regulate mitophagy, emerging data suggest that defects in mitophagy are unlikely to be of pathological relevance. However, compelling evidence to causatively link specific PINK1/parkin dependent mitochondrial pathways to dopamine neuron degeneration in PD is lacking. Evidence placing PINK1 and parkin in common pathways regulating multiple aspects of mitochondrial quality control is burgeoning. Mutations in PINK1 and parkin cause autosomal recessive Parkinson’s disease (PD).